presto
0.5.13
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Roche 454 BCR mRNA with Multiplexed Samples
Illumina MiSeq 2x250 BCR mRNA
UMI Barcoded Illumina MiSeq 2x250 BCR mRNA
UMI Barcoded Illumina MiSeq 325+275 paired-end 5’RACE BCR mRNA
Fixing UMI Problems
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Roche 454 BCR mRNA with Multiplexed Samples
Overview of Experimental Data
Overview of the Workflow
Quality control
Sample barcode and primer identification
Deduplication and filtering
Output files
Performance
Illumina MiSeq 2x250 BCR mRNA
Overview of Experimental Data
Overview of the Workflow
Paired-end assembly
Quality control and primer annotation
Deduplication and filtering
Output files
UMI Barcoded Illumina MiSeq 2x250 BCR mRNA
Overview of Experimental Data
Overview of the Workflow
Quality control, UMI annotation and primer masking
Generation of UMI consensus sequences
Paired-end assembly of UMI consensus sequences
Deduplication and filtering
Output files
Performance
UMI Barcoded Illumina MiSeq 325+275 paired-end 5’RACE BCR mRNA
Overview of Experimental Data
Overview of the Workflow
Quality control, UMI annotation and primer masking
Generation of UMI consensus sequences
Paired-end assembly of UMI consensus sequences
Deduplication and filtering
Output files
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